Scaling PacBio Sequencing with Library Prep Automation

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"As an early collaborator with Volta, we had the opportunity to observe the capabilities and speed of this technology firsthand," said Robert Sebra, PhD, Director of the Center for Advanced Genomics Technology at the Icahn School of Medicine at Mount Sinai. "We're excited to integrate the Callisto instrument into our lab, particularly given the improvements in efficiency and accelerated progress the technology has brought to our molecular pipeline, especially in supporting PacBio sequencing."

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The Callisto™ Sample Prep System has begun shipping to the first set of customers, and our team at Volta Labs is thrilled to install the very first Callisto at the Icahn School of Medicine at Mount Sinai. Check out the official announcement to learn more about Volta's first customer shipments.

The team at Mount Sinai is utilizing Volta’s novel digital fluidics platform to improve sample prep efficiency in their lab, generating PacBio libraries quickly and effectively while maintaining yield and sequencing quality. One of the Volta Apps available on Callisto is VoltaPrep™ for PacBio™ SMRTbell Libraries, which offers scalable library prep solutions for projects and of all sizes. Callisto supports flexible batch sizes up to 24 reactions with preloaded workflows to maximize Revio utilization. PacBio preps on Callisto deliver high-quality HiFi libraries that match or exceed sequencing and variant calling metrics achieved with the standard manual protocol.

Don't miss this webinar: Scaling PacBio Sequencing with Automated Library Prep for Core Labs

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On October 8, 2024, Volta Labs will be sponsoring a webinar featuring guest speakers from Mount Sinai and PacBio. In this webinar, Maya Fridrikh, PacBio sequencing manager at Mount Sinai, along with Greg Young, senior product manager at PacBio, will share data and use cases based on their experience with the Callisto Sample Prep System, which enables labs to maximize sample throughput with preloaded apps that require no user programming or optimization. Long-read SMRT (single-molecule real-time) sequencing on the PacBio platform is widely used for applications that are challenging with short-read NGS, and producing robust libraries requires precision and expertise. With the recent advancements in the throughput and speed of PacBio HiFi sequencing on the Revio, labs need reliable, efficient sample prep methods to maximize sequencing capabilities. Callisto uses advanced technology to solve scalability, flexibility, and robustness challenges in PacBio library preparation.

Key takeaways:‍

• Volta’s miniaturized PacBio app accepts the full-range of DNA mass with no loss of sequencing quality
• Users can be trained in minutes to generate PacBio libraries with minimal inter-operator variability
• Understand Callisto's compatibility with multiple shearing and size selection methods

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