Oxford Nanopore Technologies LSK114 Library Prep, automated on Callisto™
The Oxford Nanopore Technologies LSK114 DNA Library Prep App converts high molecular weight DNA into Oxford Nanopore-compatible, sequencing-ready libraries at a miniaturized reaction scale, for whole-genome, targeted and metagenomics applications.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.
How it runs on Callisto
Load
Load HMW DNA with Ligation Sequencing Kit V14 and the NEBNext Companion Module v2.
Prep on deck
Callisto runs the library prep walk-away, with a gentle process that preserves input DNA length.
Libraries out
Sequencing-ready libraries at a miniaturized reaction scale.
Sequence
Load on MinION or PromethION flow cells.
Run a new app on the Callisto™ you already have.
The protocol is already validated and automated.
Load the kit and walk away.
Performance data
Miniaturized LSK114 preps with high yields
Callisto generates significantly higher library yields across a wide range of DNA input sizes compared to manual preps. 1 µg of input DNA yields enough library to load a PromethION flowcell and generate high quality sequencing data. Key benefits also include 2x higher library yield, 2x miniaturization and up to 6x less hands-on time.
Libraries retain integrity of input DNA
The App uses a gentle process that prevents shearing, preserving the integrity of high molecular weight input DNA in the output libraries and enabling high N50 values when sequenced. The size distribution of input DNA and output libraries demonstrates strong overlap with only minimal shift.
High quality sequencing on Oxford Nanopore sequencing platforms
Libraries prepared on Callisto deliver high-quality sequencing results on MinION and PromethION platforms with exceptional scores on key metrics including N50, Q score, and pore occupancy with single loading. Consistently generating >90 GB of data is now feasible on PromethION flowcell due to the high efficiency library preparation process. Data was obtained on a PromethION flowcell that was loaded once with 400 ng of the libraries prepared on Callisto. Maximize output: achieve >90 Gb on PromethION from a single library load over 72 hours.
App note: DNA Library Prep for Oxford Nanopore LSK114.
Frequently asked questions
What DNA input does the LSK114 App need?
1 µg, or 100–200 fmol, of high molecular weight DNA.
How much hands-on time does LSK114 library prep take on Callisto?
Less than 30 minutes.
How many samples can I prep per run?
Flexible batch sizes from 4 to 24 reactions in a single run.
What applications does the app support?
Whole-genome sequencing, targeted sequencing and metagenomics on Oxford Nanopore sequencing platforms.
See Oxford Nanopore LSK114 DNA Library Prep on a Callisto in your lab.
Book a demo, or browse the full Callisto app menu.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.