IDT xGen™ v2 Hybridization Capture, automated on Callisto™
The IDT xGen v2 Hybridization Capture App automates hybridization, capture, washes and PCR setup on Callisto, delivering amplification-ready libraries for targeted sequencing.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.
How it runs on Callisto
Load
Load pooled DNA libraries, xGen panels and xGen Hybridization and Wash v2 reagents.
Hybridize
Callisto runs the hybridization step walk-away.
Capture and wash
Target capture and washes run walk-away on deck.
PCR setup
Callisto sets up PCR, ready for amplification.
Run a new app on the Callisto™ you already have.
The protocol is already validated and automated.
Load the kit and walk away.
Performance data
Uniform on-target coverage independent of operator
Callisto delivers high uniformity and on-target rates. This maximizes use of sequencing reads for better coverage of target regions, reducing the need for excessive sequencing depth and lowering overall costs.
Plexing with up to 20 libraries without loss in uniformity
Pooling more libraries into a single hybridization capture reaction on Callisto allows for greater throughput without compromising on uniformity and on-target percentages. Up to 480 libraries can be processed simultaneously using an exome panel.
Excellent target coverage for difficult to capture regions (RB1 and TERT)
RB1 and TERT are difficult to capture cancer associated genes due to their high GC content and repetitive sequences. Hybridization capture on Callisto yields higher than 90% on-target percentages with high depth of coverage in these two gene regions of interest, with minimal or no dropouts for both high GC and low GC regions.
Accurate detection of low frequency variants with Callisto over manual preps
The EGFR rs121434568 C>T SNP occurs at a low expected frequency of 1% in the Horizon cfDNA reference sample. Hybridization capture on Callisto accurately identified the C>T variant at the expected frequency of 1%.
App note: IDT xGen NGS Hyb Capture.
Frequently asked questions
Which steps does the IDT xGen App automate?
Hybridization, capture, washes and PCR setup.
How many libraries can I plex per reaction?
Flexible multiplexing from 1 to 20 libraries per reaction, with 4 to 24 reactions per run.
What input does the app need?
500 ng to 10 µg of DNA libraries.
How much hands-on time does it take?
Less than 30 minutes of set-up time.
See IDT xGen v2 Hybridization Capture on a Callisto in your lab.
Book a demo, or browse the full Callisto app menu.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.