Hybridization Capture for IDT xGen™

Overview

Workflow and Specs

Data

Ordering

Resources

Simplify workflow with walk-away, end-to-end hybridization capture

Volta's Hybridization Capture for IDT  xGen™ offers fully automated hybridization, capture, washes, and PCR setup to deliver high-quality libraries without inter-operator variability. Allows you to process up to 480 exomes per run.

Key benefits

  • Decrease your sequencing redo rates to less than 2%
  • Achieve uniform coverage across high- and low-GC content regions
  • Save on probe costs by plexing up to 20 libraries

ACCESS THIS APP ON CALLISTO

get in touch

The IDT xGen NGS Hyb Capture workflow is designed to provide consistent coverage uniformity and a high proportion of on-target bases for a broad range of panel sizes. Callisto's walk-away automation supports flexible batch sizes from 4-24 reactions per run with high-quality enrichment performance independent of the operator. With less than 30 minutes of set-up time, you'll expedite the output of amplification-ready libraries. High-throughput projects are also enabled through sample multiplexing, from 1-20 plex per reaction.

Whether you want to run the hybridization overnight or on the same day, Volta's IDT xGen NGS Hyb Capture app will accommodate your project needs.

App Specifications

Input: 500 ng to 10µg of DNA libraries
Output: Amplification libraries ready to sequence
Hands-on time: Less than 30 mins
Callisto processing time: 21 hours
Throughput: Flexible, 4 to 24 rxns in a single run
Multiplexing: Flexible, 1-20 libraries
Panel Size: ~10k (Pan-Cancer) to ~400k (Exome) probes
Chemistry: IDT xGen™ Hybridization Capture (HC0001)

ACCESS THIS APP ON CALLISTO

get in touch

Uniform on-target coverage independent of operator

Callisto delivers high uniformity and on-target rates. This maximizes use of sequencing reads for better coverage of target regions, reducing the need for excessive sequencing depth and lowering overall costs.

Plexing with up to 20 libraries without loss in uniformity

Pooling more libraries into a single hybridization capture reaction on Callisto allows for greater throughput without compromising on uniformity and on-target percentages. Up to 480 libraries can be processed simultaneously using an exome panel. Higher throughput in combination with significantly reduced hands-on time leads to considerable cost savings.

Excellent target coverage for difficult to capture regions (RB1 and TERT exon)

RB1 and TERT are difficult to capture cancer associated genes due to their high GC content and repetitive sequences. Hybridization capture on Callisto yields higher than 90% on-target percentages with high depth of coverage in these two gene regions of interest, with minimal or no dropouts for both high GC and low GC regions.

Accurate detection of low frequency variants with Callisto over manual preps

The EGFR rs121434568 C>T SNP occurs at a low expected frequency of 1% in the Horizon cfDNA reference sample. Hybridization capture on Callisto accurately identified the C>T variant at the expected frequency of 1%.

ACCESS THIS APP ON CALLISTO

get in touch
To process samples using Hyb Capture for IDT xGEN App, you will need the following materials:
Volta Labs
Hyb Capture for IDT xGen™ (16 rxns)
HC0001
IDT DNA*
xGen Hybridization and Wash v2 Reagents, 16 reactions kit
10010351
xGen Hybridization and Wash v2 Beads, 16 reactions
10010353
Streptavidin Beads
10010353
xGen™ Predesigned Hybridization Capture Panels
NA
xGen Universal Blockers TS, 16 reactions
1075474
xGen Library Amplification Primer Mix, 16 reactions
1077675
Beckman Coulter*
AMPure XP beads
A63880
*Please contact our partners directly to purchase these reagents.
To order: sales@voltalabs.co

ACCESS THIS APP ON CALLISTO

get in touch
Recorded webinar

Revolutionizing Genomic Sample Prep with Volta and IDT
poster
Automated xGEN™ NGS Hybridization Capture and Wash Steps on the Volta Labs Digital Fluidics Platform Improves Consistency and Reduces Hands-on Time
Data sheet
App Notes
IDT xGen NGS Hyb Capture

IDT Collab Network Partner

Volta Labs has been recognized by IDT in the IDT Collab Network as an expert in the field of NGS sample prep. Volta and IDT are working to create platform-agnostic solutions with the goal of walk-away sample prep, starting with target enrichment workflows and Volta's IDT xGen NGS Hyb Capture app for Callisto. Together, this partnership will accelerate the journey from sample to data, enabling rapid, high-quality processing for as little as one sample.

About the partnership


ACCESS THIS APP ON CALLISTO

get in touch