High-molecular weight (HMW) DNA Extraction
Isolate HMW DNA with ease from a variety of samples types
Volta's HMW DNA Extraction App offers purification of HMW DNA using a fully automated magnetic bead-based method on Callisto. The App is compatible with whole blood with additional sample types to be supported over time. DNA extracted with this App can be used with PacBio and Oxford nanopore sequencing platforms.
KEY BENEFITS
- Get 3 hours of walkaway time with Callisto’s gentle processing
- Operationalize HMW DNA extractions in your lab
- Produce fragment lengths >40 kb consistently with high yields
Volta's HMW DNA Extraction App offers purification of HMW DNA using a fully automated magnetic bead-based method on Callisto. The App is compatible with whole blood with additional sample types to be supported over time. DNA extracted with this App can be used with PacBio and Oxford nanopore sequencing platforms.
App Specifications
Input: 200 µL whole blood
Output: Greater than 2 µg HMW DNA for whole blood with 6x10^6 WBC/mL
Sample type: Whole blood, Buffy coat and Cell lines
Hands-on time: Less than 45 mins
Walk-away time: 3.5 hours
Throughput: Flexible, 4 to 24 rxns in a single run
Chemistry: VoltaPure™ for High-Molecular Weight (HMW) DNA extraction
High yields across diverse donors
The total DNA yield is a function of WBC count. The HMW DNA extraction app consistently delivers >1 µg of high-quality HMW DNA from samples with >6 million cells/mL, providing sufficient input for long-read sequencing. DNA concentration was measured using Qubit dsDNA HS.
DNA consistently meets fragment length and purity specs for all downstream library preps.
Callisto platform produces HMW DNA with high purity and long fragment length. Purity (Left): A260/280 ratio consistently falls within the 1.7 – 2.2 range (600 samples) indicating no protein contamination. Fragment length (Right): Genomic Quality Number (GQN) represents the percentage of a sample's total DNA that exceeds a specified size threshold, indicating its suitability for long-read sequencing. Callisto platform produces GQN10Kb score of >9 (90% of fragments are above 10 kb) and GQN40Kb >6 (60% of fragments are above 40 kb) .
Achieve industry-leading fragment lengths with minimal hands-on time
Callisto outperforms five widely used HMW DNA extraction kits across multiple chemistries—including magnetic bead, disc, glass bead, column, and PEG-based volume exclusion methods. While yields (normalized yields per microliter) and purity are comparable, Callisto delivers significantly longer DNA fragments with far less hands-on time. Manual methods require 3–6x more hands-on time, making Callisto the superior choice for efficient, high-quality extractions.
High-quality PacBio sequencing using HMW DNA extracted on Callisto
PacBio sequencing was performed on libraries prepared using VoltaPure HMW DNA extraction and PacBio library prep Apps on Callisto. Key sequencing metrics—including mean read length, N50, and read length distribution meets PacBio sequencing specifications—demonstrates DNA extracted on Callisto is suitable for PacBio sequencing. Similar HiFi read lengths distribution across replicates, highlighting the reproducibility. Blue pippin was used to remove fragments <10 kb for optimal sequencing.
To process samples using High-molecular weight (HMW) DNA Extraction you will need the following materials:
Volta Labs
HMW DNA Extraction (96 rxns)
EX0001
To order: sales@voltalabs.co
