PacBio SMRTbell 3.0 Library Prep

Overview

Workflow and Specs

Data

Ordering

Resources

Maintain rapid turnaround time through flexible batch sizing

Volta's PacBio™ SMRTbell 3.0 Library Prep App offers an automated process of converting DNA into SMRTbell libraries, at a miniature reaction scale. The process includes end repair, ligation, nuclease treatment and optional size selection. The App can be used for de novo genome assembly, variant detection, AAV sequencing and amplicon sequencing.

KEY BENEFITS

  • Get 5.5 hours of walkaway time for 24 samples
  • Prep 2x more reactions from library prep kit
  • Achieve the highest quality libraries with no prior experience preparing libraries

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The PacBio SMRTbell 3.0 Library prep app offers robust and versatile library preparation through preloaded apps for high-quality HiFi long-read sequencing. Callisto's walk-away library prep automation supports flexible batch sizes from 4-24 reactions per run with less than 30 minutes of set-up time. Gain 5.5 hours of walk-away time for every 24 sample run performed on Callisto.



App Specifications

Input: 1 - 5 µg DNA
Output: HiFi libraries for Revio/Sequell IIe systems
Hands-on time: Less than 30 mins
Walk-away time: 5.5 hours
Throughput: Flexible, 4 to 24 rxns in a single run
Chemistry: PacBio SMRTbell prep kit 3.0 (102-182-700) with bead-based size selection

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Miniaturized PacBio preps without compromising on performance

Miniaturized half-scale PacBio libraries prepared on Callisto (blue) yield equivalent final library masses to full-scale manual preps (grey). Library yield scales reliably with input gDNA (1–5 µg), demonstrating robust and reproducible performance across a broad input range.

Consistent, high-quality HiFi sequencing on Revio

Callisto-prepped libraries deliver high-quality HiFi sequencing results on PacBio Revio, performing on par with or better than manual preparations. Key sequencing metrics—including HiFi read N50, total read count, and Q score—are maintained with high consistency, ensuring reliable and high-fidelity data with every run. Data presented includes 12 manually prepared libraries and 24 libraries processed using Callisto.

Robust variant calling performance

Callisto-prepped libraries (blue) deliver highly accurate variant calling results, matching or exceeding manual preps (grey) in SNP and INDEL precision, recall, and F1 scores. Libraries were prepared using HG001 reference DNA and sequenced on PacBio Revio. Variant calling was performed with Google DeepVariant and benchmarked against hg38 NIST v4.2.1 using hap.py.

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To process samples using PacBio SMRTbell 3.0 Library Prep you will need the following materials:
Volta Labs
PacBio SMRTbell 3.0 Library Prep (48 rxns)
LP0001
PacBio*
PacBio SMRTbell prep kit 3.0
102-141-700
PacBio AMPure® PB beads
100-265-900
PacBio PacBio Elution Buffer#
101-633-500
PacBio SMRTbell cleanup beads#
102-158-300
Low TE Buffer pH 8.0#
102-178-400
*Please contact our partners directly to purchase these reagents.
#The SMRTbell prep kit 3.0 does not include SMRTbell cleanup beads, AMPure® PB beads, low TE Buffer and a sufficient quantity of PacBio Elution Buffer to perform sample preparation. Purchasing these reagents separately from PacBio is required.
To order: sales@voltalabs.co

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Recorded webinar

Scaling PacBio sequencing with automated library prep
poster
Assessment of a Novel Digital Fluidics Platform for Rapid Sample Processing from Whole Blood to Library
poster
Digital Fluidics Enables Broad-based Access to Push-button NGS Sample Prep
Data sheet
 PacBio SMRTbell 3.0 Library Prep

PacBio Compatible Partner

Volta Labs is partnering with PacBio to offer walk-away automation for PacBio library prep, enabling labs to scale sample prep for sequencing. Through preloaded apps on the Callisto Sample Prep System, PacBio customers are able to prepare high-quality PacBio libraries with less than 30 minutes of hands-on time and 5.5 hours of walk-away time. Users can be trained in minutes to generate PacBio libraries with minimal inter-operator variability.


Learn more about Volta and the PacBio Compatible Program

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