DNA Library Prep for Illumina  

Highly reproducible Illumina libraries

Volta's DNA Library Prep for Illumina™ offers an automated, streamlined process for efficiently converting DNA of variable quantities into Illumina™ compatible sequencing ready libraries. The process includes enzymatic fragmentation, end-repair, A-tailing, adapter ligation and bead-based size selection, producing libraries with minimal bias and artifacts. The App supports whole genome sequencing, whole exome sequencing, and targeted sequencing by offering different library fragment sizes.

key benefits

Delivers consistent fragment lengths
Low duplication rates of <10%
Robust workflow with < 1 in 300 failure rate

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Volta's DNA Library Prep for Illumina™ offers scalable library prep solutions for projects and genomes of all sizes. Callisto supports flexible batch sizes up to 24 reactions with preloaded workflows to enable Illumina sequencing. Fully automated library prep on Callisto delivers high-quality Illumina libraries, enabling Illumina library preparation for multiple platforms like the NovaSeq X/X Plus and the NextSeq 1000/2000.

App Specifications

Input: 50-500 ng DNA

Output: Sequencing ready dsDNA libraries

Hands-on time: Less than 30 mins

Walk-away time: 3 hours

Throughput: Flexible, 4 to 24 rxns in a single run

Chemistry: VoltaPrep™ Enzymatic Fragmentation - Illumina (LP0002)

ACCESS THIS APP ON CALLISTO

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Highly reproducible output library size across input sources and masses

Callisto demonstrated highly reproducible library size, consistently achieving approximately 750 bp mode library size across four distinct genomic DNA sources suitable for whole genome sequencing. These sources included two human reference genomes (HG001, HG002), bacterial DNA (ZymoBIOMICS Microbial Community Standard), and DNA extracted from whole blood (WB). Additionally, Callisto produced consistent library size across a broad input DNA mass range from 100 ng to 500 ng, ensuring robust performance and reliability for diverse sample types.

Compatible with wide input DNA range

Callisto generated library concentrations exceeding 2 nM with input DNA inputs as low as 100 ng. The system also demonstrated compatibility with higher input amounts, supporting DNA masses up to 500 ng, ensuring flexibility across a broad range of sample inputs.

Robust variant calling performance

Callisto-prepped libraries deliver highly accurate variant calling results across multiple technical replicates (n=5) in SNP and INDEL precision, recall, and F1 scores. Libraries were prepared using HG002 reference DNA and sequenced on Illumina NovaSeq X. Variant calling was performed with NVIDIA Parabricks 4.4.0.1 and benchmarked against the most recent NIST benchmark (HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed) using hap.py v0.3.12.