DNA EF Library Prep (powered by Watchmaker), automated on Callisto™
This app converts DNA into Illumina-compatible, sequencing-ready libraries on Callisto, for PCR-free whole-genome sequencing, whole-exome sequencing and targeted sequencing.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.
How it runs on Callisto
Load
Load DNA and the Callisto Complete Kit for DNA EF Library Prep.
Fragment and A-tail
Enzymatic fragmentation, end repair and A-tailing run walk-away.
Ligate and size-select
Adapter ligation and bead-based size selection on deck.
Libraries out
Sequencing-ready libraries for Illumina, UG 100 or Element AVITI.
Run a new app on the Callisto™ you already have.
The protocol is already validated and automated.
Load the kit and walk away.
Performance data
Highly reproducible output library size across input sources and masses
Callisto generates libraries that are suitable for whole genome sequencing. The library prep App consistently delivers mode library size of approximately 600 bp across a broad input DNA mass range from 100ng-500ng.
Compatible with a wide input DNA range
Callisto generates library concentrations exceeding 2 nM (with qPCR) with input DNA amounts as low as 100 ng. The system also demonstrates compatibility with higher input amounts, supporting DNA masses up to 500 ng, ensuring flexibility across a broad range of sample inputs.
Equivalent yield from various DNA sources
The App is compatible with various input DNA sources yielding about the same concentration of libraries across the DNA types. The output concentrations exceed 2 nM in all cases. The DNA sources include two human reference genomes (HG001, HG002), bacterial DNA (ZymoBIOMICS Microbial Community Standard), and DNA extracted from whole blood (WB).
Robust variant calling performance
Callisto-prepped libraries deliver highly accurate variant calling results across multiple technical replicates (n=5) in SNP and INDEL precision, recall, and F1 scores. Libraries were prepared using HG002 reference DNA and sequenced on Illumina NovaSeq X. Variant calling was performed with NVIDIA Parabricks 4.4.0.1 and benchmarked against the most recent NIST benchmark (HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed) using hap.py v0.3.12.
Low duplication rates and production-ready reliability
The App delivers exceptionally low duplication rates (<10%) and a fully automated walk-away workflow with a failure rate of <1 in 300 runs. Hands-on time is less than 30 minutes, with 3 hours of walk-away time for every 24-sample run.
Source: results previously published on this page. See also the DNA EF Library Prep (powered by Watchmaker).
Frequently asked questions
Which sequencers is DNA EF Library Prep on Callisto compatible with?
Illumina sequencers, UG 100 and Element AVITI.
How much hands-on time does it take?
Less than 30 minutes of set-up time per run.
How many samples can I prep per run?
Flexible batch sizes from 4 to 24 reactions in a single run.
What applications does the app support?
PCR-free whole-genome sequencing, whole-exome sequencing and targeted sequencing, by offering different library fragment sizes.
See DNA EF Library Prep (powered by Watchmaker) on a Callisto in your lab.
Book a demo, or browse the full Callisto app menu.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.