Twist Standard Hyb v2, automated on Callisto™
The Twist Standard Hyb v2 App on Callisto automates hybridization capture with Twist Bioscience chemistry, from input libraries to enriched, PCR-ready libraries.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.
How it runs on Callisto
Set up
Set up the hybridization reactions at the bench.
Hybridize
Callisto runs the overnight hybridization step.
Capture and wash
Load capture beads and wash buffers; Callisto automates target capture, heated washes and PCR setup.
Amplify
Take libraries off for PCR, then optionally return them for automated post-PCR cleanup.
Run a new app on the Callisto™ you already have.
The protocol is already validated and automated.
Load the kit and walk away.
Performance data
High quality sequencing metrics
Callisto delivers high uniformity and on-target rates comparable or better than the manual workflow. The data presented here is using the Twist Exome 2.0 panel downsampled to 50 M reads.
Uniform coverage across targets
Callisto delivers unbiased coverage across a wide range of GC contents on par with manually processed samples. The data presented here uses the Twist Exome 2.0 panel.
Excellent target coverage for difficult to capture regions (ACTA1 and RASA4B)
ACTA1 and RASA4B are select examples of difficult to capture genes that are systematically undercovered. Hybridization capture on Callisto yields high depth of coverage in these gene regions of interest, with minimal or no dropouts for both high GC and low GC regions.
Frequently asked questions
What does the Twist Standard Hyb v2 App automate?
Overnight hybridization, target capture, heated washes and PCR setup, from input libraries to enriched, PCR-ready libraries.
What input does the app need?
500 ng to 1.5 µg of DNA libraries, with flexible multiplexing of 1 to 8 libraries.
How many reactions can I run?
4 to 24 reactions in a single run.
How much hands-on time does it take?
Less than 30 minutes.
See Twist Standard Hyb v2 Hybridization Capture on a Callisto in your lab.
Book a demo, or browse the full Callisto app menu.
Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.