The Callisto app menu

One instrument. A growing app menu.

14 released apps on the same Callisto™: extraction, library prep and hybrid capture, across short reads and long reads. Add a chemistry by loading a kit, not buying another box.

AXELIOS 1
Illumina
Oxford Nanopore Technologies
PacBio
Element
Ultima Genomics

For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.

14 released apps, one instrument
The menu keeps growing
A new chemistry is a kit, not a new system
What’s an app?

Trying a new chemistry or sequencer is as simple as buying the kit.

✓

No new instrument

Run a new app on the Callisto™ you already have.

✓

No re-automation project

The protocol is already validated and automated on Callisto.

✓

No new method development

Load the kit and walk away. No programming and no protocol development on your side.

An app is a validated protocol and the reagents you load on the same Callisto™. One instrument moves from extraction to library prep to hybrid capture, across Illumina, Oxford Nanopore, PacBio and AXELIOS 1 sequencers.

01

Pick an app

Choose a validated workflow already on the platform: the chemistry you run today, or the one you want to try next.

02

Load the kit

Load the Callisto kit and the chemistry provider’s reagents. No programming and no protocol development on your side.

03

Walk away

Callisto runs the app. The same instrument is ready for a different app on the next run.

The menu keeps growing

New on Callisto: AXELIOS 1 Library Prep, in collaboration with Roche. More validated apps continue to be added to the same instrument.

For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.

Featured

Two apps we’re highlighting now.

New · In collaboration with Roche

AXELIOS 1 Library Prep

50 ng of purified DNA in, sequencing-ready libraries out, on Callisto. Pre-optimized, so there’s no protocol development or tuning.

Hands-on time
~1 h (45 min setup + 15 min touchpoint)
Batch size
4 to 24 samples per run
Run time
16 hours
Sequencer
AXELIOS 1
View app →
For Research Use Only. Not for use in diagnostic procedures.
Library prep

Oxford Nanopore Technologies LSK114

Automated ligation sequencing library prep on Callisto, alongside high molecular weight DNA extraction on the same instrument.

Hands-on time
Less than 30 minutes
Batch size
4 to 24 reactions per run
Walk-away time
3.5 hours
Input
1 µg, or 100–200 fmol of HMW DNA
View app →
For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.
App library

All 14 released apps, in one place.

Grouped by workflow step. Each card links to its app page.

Extraction 2 apps

Extraction

HMW DNA extraction

PacBio · Oxford Nanopore
Input
200 µL whole blood; also buffy coat and cell lines
Batch size
4 to 24 reactions
View app →
Extraction

DNA extraction for short-read sequencing

Short-read sequencing

DNA extraction for short-read sequencing workflows. See the app page for details.

View app →

Library prep 9 apps

Library prep

AXELIOS 1 Library Prep

AXELIOS 1 · In collaboration with Roche
Input
50 ng purified DNA
Hands-on
~1 hour
Batch size
4 to 24 samples
View app →
Library prep

Oxford Nanopore LSK114

Oxford Nanopore
Input
1 µg, or 100–200 fmol of HMW DNA
Hands-on
Less than 30 minutes
Batch size
4 to 24 reactions
View app →
Library prep

PacBio SMRTbell® 3.0

PacBio
Input
1–5 µg DNA
Batch size
4 to 24 reactions
View app →
Library prep

Watchmaker DNA EF

Illumina · UG 100 · Element AVITI
Hands-on
Less than 30 minutes
Batch size
4 to 24 reactions
View app →
Library prep

KAPA EvoPlus Boost

Illumina-compatible · Roche chemistry (RUO)
Hands-on
Less than 30 minutes
Batch size
4 to 24 reactions
View app →
Library prep

Illumina DNA PCR-Free

Illumina

Part of the Callisto DNA library prep menu.

View library prep menu →
Library prep

NEB Ultra II FS

Illumina · Ultima · Element

Part of the Callisto DNA library prep menu.

View library prep menu →
Library prep

Twist EF 2.0

Illumina · Ultima · Element

Part of the Callisto DNA library prep menu.

View library prep menu →
Library prep

QIAseq FX

Illumina · Ultima · Element

Part of the Callisto DNA library prep menu.

View library prep menu →

Hybrid capture 3 apps

Hybrid capture

IDT xGen™ Hybrid Capture

Targeted sequencing
Input
500 ng to 10 µg of DNA libraries
Hands-on
Less than 30 minutes
Batch size
4 to 24 reactions · 1–20 plex
View app →
Hybrid capture

Twist Standard Hyb v2

Targeted sequencing
Input
500 ng–1.5 µg DNA libraries
Hands-on
Less than 30 minutes
Batch size
4 to 24 reactions · 1–8 plex
View app →
Hybrid capture

Twist Fast Hyb Cap

Targeted sequencing
Input
500 ng–1.5 µg DNA libraries
Hands-on
Less than 30 minutes
Batch size
4 reactions per run · 1–8 plex
View app →
Also on Callisto: a tool. Double-sided size selection is listed as a Callisto tool rather than an app, so it isn’t counted in the 14.

For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.

See the menu on a Callisto in your lab.

Book a demo, or start with the instrument if you want the full picture.

join the journey

discover the ease of push-button sample prep