In this study, we investigated the feasibility and potential benefits of the Volta Labs™ automated sample prep platform for a clinical setting using an established commercial library preparation method for PCR-free WGS. The overall quality of libraries prepared at half scale on the Volta Labs platform matched or exceeded that achieved with the full-scale manual protocol. This study provides a foundation for validating the performance of the Callisto™ Sample Prep System with larger cohorts of clinically relevant samples.